Past Issues

2024: Volume 3, Issue 1

Effect of Dexamethasone in Feed on Intestinal Permeability, Differential White Blood Cell and Immune Organs in Rabbits

Allah Bux Kachiwal1,*, Saeed Ahmed Soomro1, Shamsuddin Bughio2, Rabia Saeed1 Rajput, Aliza Memon1, Jamil Ahmed Shaikh1

1Department of Physiology and Biochemistry, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, Pakistan

2Department of Pharmocology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, Pakistan

*Corresponding Author: Allah Bux Kachiwal, Department of Physiology and Biochemistry, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, Pakistan; Email: [email protected]

Received Date: February 25, 2024

Publication Date: March 25, 2024

Citation: Kachiwal AB, et al. (2024). Effect of Dexamethasone in Feed on Intestinal Permeability, Differential White Blood Cell and Immune Organs in Rabbits. Gastro Res. 3(1):7.

Copyright: Kachiwal AB, et al. © (2024). 

ABSTRACT

An experiment was conducted to evaluate the effect of stress on intestinal permeability induced by administration of dexamethasone in feed. Male albino rabbits (n=10) was purchased from local market of Hyderabad and brought to Animal House, FAHVS SAU Tandojam. Rabbits were assigned to the two groups; Group A (control) and group B (dexamethasone fed). Rabbits were kept in a monitored facility with full access to food and water that was age-appropriate.  For the rest of the trial, the DEXF treatment group was switched to feed containing DEXF1X (0.57 ppm) on day 3. The results revealed that dexamethasone-fed rabbits (Group B) exhibited a significant decrease in body weight (2104±28.66g) compared to the control group (Group A) (2506±45.81g). The serum fluorescein Isothiocyanate dextrin level in group -B was significantly ( P<0.05) Higher (0.29±0.3 kg/ml) than that of rabbits in group A ( control ) with mean serum FTTC-D (0.22±0.01 kg/ml) Total liver aerobic bacteria in group -B supplemented dexamethasone fed was significantly ( P<0.05) Higher (1.2± 0.45log10 Cfu/g) than that of rabbits in group -A (control) with mean total liver aerobic bacteria (0.13 ± 0.05 Log10 cfu/g ). The WBC count was significantly higher in Group B (9.74±1.22 ×10^3/µL) than in Group A (7.02±0.48 ×10^3/µL). Heterophils count was significantly elevated in Group B (40.44±7.42%) compared to Group A (25.08±5.32%). Lymphocytes count was significantly lower in Group B (46.12±5.32%) compared to Group A (58.12±4.67%). Monocytes count showed no significant difference between the groups, with Group A (8.26±1.47%) being slightly higher than Group B (6.96±1.83%). Eosinophils count was significantly lower in Group B (3.70±1.22%) compared to Group A (5.72±1.07%). Basophils count showed no significant difference between the groups, with Group A (2.84±0.57%) being slightly higher than Group B (2.68±0.30%). The H/L ratio was significantly higher in Group B (0.87±0.02) than in Group A (0.43±0.01). Effect of dexamethasone on lymph nodes, thymus, spleen and Intestine Morphology was observed through histological study. Thymic lobule length was significantly lower in Group B (380±2.30 µm) compared to Group A (520±4.97 µm). Thymic lobule width was significantly lower in Group B (180±1.73 µm) compared to Group A (240±2.66 µm). White pulp length was significantly lower in Group B (340±6.14 µm) compared to Group A (470±7.27 µm). White pulp width was significantly lower in Group B (140±1.74 µm) compared to Group A (220±3.82 µm). These findings indicate that dexamethasone administration in the feed of rabbits leads to a decrease in body weight, altered WBC count, increased heterophils count, decreased lymphocytes count, reduced eosinophils count, and an elevated H/L ratio. Additionally, dexamethasone adversely affects thymic lobule length and width, as well as white pulp length and width in the thymus and spleen, respectively.

Keywords: Blood profile, Spleen histology, Dexamethazone, Thymus, Body weight, Rabbit

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